The Effect of Progesterone on the Viability of MCF-7 Cell Line and Evaluation of Expression of P53, BAX and BCL-2 Genes

Background and Objectives: Researches have shown that progesterone influences the viability of breast cancer cells. The purpose of this study was to investigate the effect of progesterone on the viability of MCF-7 cell line and evaluation of Expression level of P53, Bax, and Bcl-2 Genes. Methods: In this laboratory-experimental study, MCF-7 cells were purchased from Pasture institute and divided into control group and the group received progesterone in the concentrations of 0.001, 0.01, 0.1, 1, and 10mg/mL. The viability of the cells was assessed using MTT assay. The relative expression level of P53, Bax, and Bcl-2 genes was investigated by Real Time PCR technique. Data analysis was performed using one-way ANOVA and independent samples t-test. Results: Exposure to 10mg/ml of progesterone resulted in decreased viability of MCF-7 cells compared to the control group (p