Long-term culture of rat hepatocytes using human amniotic membrane as a culture substrate

Amniotic membrane is attracting attention as a new material for regenerative medicine. We herein report that the culture of primary rat hepatocytes on human amniotic membrane maintained their morphology and their production of albumin for at least two months. Human amniotic membrane was collected during planned cesarean section and kept frozen until usage. Primary rat hepatocytes were plated on human amniotic membrane. Hepatocytes accumulated as colonies on amniotic membrane, and their rat albumin level was maintained for two months. Their three-dimensional structure on extracellular matrix, which is abundant in amniotic membranes might influence the maintenance of the hepatocyte-specific function. •Long-term primary culture of rat hepatocyte on the human amniotic membrane was successful.•Albumin production from primary isolated hepatocytes was maintained for the long term.•Amniotic membrane provided the situation of 3D structure for isolated rat hepatocyte.