Bifidobacterium response to lactulose ingestion in the gut relies on a solute-binding protein-dependent ABC transporter

This study aims to understand the mechanistic basis underlying the response of Bifidobacterium to lactulose ingestion in guts of healthy Japanese subjects, with specific focus on a lactulose transporter. An in vitro assay using mutant strains of Bifidobacterium longum subsp. longum 105-A shows that a solute-binding protein with locus tag number BL105A_0502 (termed LT-SBP) is primarily involved in lactulose uptake. By quantifying faecal abundance of LT-SBP orthologues, which is defined by phylogenetic analysis, we find that subjects with 10 7 to 10 9 copies of the genes per gram of faeces before lactulose ingestion show a marked increase in Bifidobacterium after ingestion, suggesting the presence of thresholds between responders and non-responders to lactulose. These results help predict the prebiotics-responder and non-responder status and provide an insight into clinical interventions that test the efficacy of prebiotics. Yoshida et al. investigate the role of an ABC transporter (LT-SBP) in lactulose metabolism and its putative role in enriching the gut microbiota with bifidobacteria that encode this transporter. Their results might help in predicting prebiotics-responder and nonresponder status, helping the clinical interventions testing the efficacy of prebiotics.