Effect of Oral Administration of Astragalus ecbatanus Chloroform Extract on Acute and Chronic Pain in Balb/C Mice

Background and objectives: Astragalus spp., have been used as a pain reliever in traditional medicine; therefore, in this study, we decided to evaluate the antinociceptive effects of Astragalus ecbatanus chloroform extract (AECE) in acute and chronic pain in male mice. Methods: The extract was obtained from aerial parts of A. ecbatanus with maceration method. The antinociceptive effect of AECE was determined by tail-flick, hot-plate, formalin, and rotarod tests followed by the oral intake of mice with AECE at the doses of 200, 400, and 800 mg/kg for 14 days in male Balb/C mice. Results: The results showed AECE at the concentrations of 400 and 800 mg/kg revealed a mean latency time of 6.4 and 7.2 s, respectively; representing a remarkable (p