C15, C20, and C25 isoprenoid homologues in glycerol diether phospholipids of methanogenic archaebacteria

C15, C20, and C25 isoprenoid homologues in glycerol diether phospholipids of methanogenic archaebacteria

The glycerol diether phospholipids of 25 monocultures of methanogenic bacteria were isolated and degraded with hydriodic acid. The resulting alkyl iodides were converted to acetate esters and alcohols which were examined using capillary gas-liquid chromatography. The presence of C20 phytanol was obs...

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Veröffentlicht in:Journal of lipid research 1985-09, Vol.26 (9), p.1120-1125
Hauptverfasser: Mancuso, C A, Odham, G, Westerdahl, G, Reeve, J N, White, D C
Format: Artikel
Sprache:eng
Schlagworte:
Archaea - analysis
Bacteria - analysis
Chromatography, Gas
Chromatography, Thin Layer
Diterpenes - analysis
Esters
Euryarchaeota - analysis
Gas Chromatography-Mass Spectrometry
Phospholipids - analysis
Plasmalogens - analysis
Sesquiterpenes - analysis
Species Specificity
Terpenes - analysis
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Zusammenfassung:The glycerol diether phospholipids of 25 monocultures of methanogenic bacteria were isolated and degraded with hydriodic acid. The resulting alkyl iodides were converted to acetate esters and alcohols which were examined using capillary gas-liquid chromatography. The presence of C20 phytanol was observed in accordance with previous studies. Soft fragmentation by chemical ionization mass spectrometry combined with selected ion monitoring enabled the detection, for the first time, of C15 and C25 isoprenologues as components of the diether phospholipids in several strains.
ISSN:0022-2275
DOI:10.1016/S0022-2275(20)34285-1