Tetra-Primer ARMS PCR and Its Applications in Genotyping Point Mutations and Single Nucleotide Polymorphisms

Single nucleotide polymorphism is the most frequent type of polymorphism in the genome sequence, mostly used as the genetic marker for identification. Moreover, point mutations are diagnosable and detectable similar to single nucleotide polymorphisms which cause genetic diseases. Thus, it is important to suggest a technique to detect many mutations with high speed and accuracy and low cost. Tetra-primer ARMS PCR is able to provide the possibility of identifying different allele forms of a single nucleotide polymorphism in a PCR reaction. In this study, two common point mutations of IVSII-I (G-A) and IVSI-110(G-A) in beta thalassemia were detected and genotyped by tetra-primer ARMS technique. Finally, accuracy and precision of this techniques were analyzed and evaluated using sequencing results. As the results of genotyping by tetra-primer ARMS and sequencing were the same, it seems that the former is capable to turn into a routine test in diagnostic laboratories and clinics.