Selenium Nanoparticles Biosynthesized by Pantoea agglomerans and Their Effects on Cellular and Physiological Parameters in the Rainbow Trout Oncorhynchus mykiss

The applications of nanoparticles (Nps) as food additives, health enhancers, and antimicrobials in animal production are increasing. The aim of this study was to evaluate the effect of selenium (Se) nanoparticles (Se Nps) stabilized with L-cysteine (Se Nps/L-Cys), as a nutritional supplement, on immunological, oxidative status, and productive parameters in TEM and SEM-EDS showed the accumulation of spherical Se Nps entirely composed by elemental selenium (Se ) as intracellular and extracellular deposits in UC-32 strain. The in vitro antioxidant capacity of Se Nps/L-Cys was significant more efficient ROS scavengers than Se Nps and Na SeO . We also evaluate the effect of Se Nps/L-Cys on cell viability and oxidative stress in RTgill-W1, RTS-11, or T-PHKM cell lines. Se Nps/L-Cys showed less toxic and high antioxidant activity than Se Nps and Na SeO . Finally, the dietary Se Nps/L-Cys had a significant better effect on both plasma lysozyme and respiratory burst activity (innate immune response), on tissular Gpx activity (oxidative status), and on well-being (productive parameter) of when it is compared to Se Nps and Na SeO . Se Nps/L-Cys is a promising alternative for nutritional supplement for with better performance than Na SeO and Se Nps, ease to implementation, and reduced environmental impact.