Isolation and characterization of Indole Acetic Acid-producing bacteria isolated from rhizospheric soil of paddy rice

Plant growth-promoting rhizobacteria have immense potential to be used as an environmentally friendly replacement for chemical fertilizers and pesticides, through the production of various plant growth-promoting substances. The objectives of this study were the isolation, characterization, and identification of indole acetic acid-producing bacteria from the rhizospheric soil of paddy rice. Out of all screened isolates, isolate 1.4 was the highest IAA producer with 29.76 µg/mL. The culture medium conditions to obtain the highest IAA production were optimized as follows: the culture temperature was 30°C, the incubation time was three days, and the pH was 7.0. The 16S rRNA gene sequence showed that isolate 1.4 has been genetically identified as Bacillus altitudinis , and it can potentially be used as an efficient biofertilizer.