Extraction Strategy for DNA Recovery from Putrefied Teeth and Skull Bone

Forensic samples are commonly exposed to harsh environmental conditions which affect the degree of sample (DNA) preservation and subsequent genetic profiling. The aim of this study was to develop a better strategy for DNA extraction from hard putrefied tissues (Teeth and Skull bone). Jaw (teeth) and...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Arab journal of forensic sciences & forensic medicine 2016, Vol.1 (4), p.399-405
Hauptverfasser: Kamoun, Arwa, Amar, Wiem Ben, Mahfoudh, Nadia, Maalej, Leila, Mallek, Bakhta, Nouma, Youssef, Bardaa, Sami, Makni, Hafedh, Maatoug, Samir
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Forensic samples are commonly exposed to harsh environmental conditions which affect the degree of sample (DNA) preservation and subsequent genetic profiling. The aim of this study was to develop a better strategy for DNA extraction from hard putrefied tissues (Teeth and Skull bone). Jaw (teeth) and the skull samples were collected from the putrefied corpses and the authors were asked to determine if the two specimens belonged to the same body. The DNA was extracted by phenol-chloroform and DNA IQ™ System Kit. The PowerPlex®  16 and the PowerPlex® Y System Kits were used for autosomal STR and Y-STR genotyping, respectively. DNA profiling found evidence in favor of DNA degradation. Phenol-Chloroform extracted-DNA was re-extracted by using DNA IQ ™ System kit and managed to identify 13 autosomal STR loci and 13 Y-STR markers from doubly extracted DNA. In conclusion, the combination of two DNA extraction methods (phenol-chloroform + DNA IQ™) improved the quality of DNA extracted from putrefied teeth and skull bone.
ISSN:1658-6786
1658-6794
DOI:10.12816/0033133