Supplementation with nanobubble water alleviates obesity-associated markers through modulation of gut microbiota in high-fat diet fed mice

Supplementation with N2-NBW effectively alleviated the obesity-associated markers in the high-fat diet fed mice. [Display omitted] •Effects of nanobubble water (NBW) on the process of high-fat diet (HFD) induced obesity was investigated for the first time.•Supplementation with nitrogen NBW alleviated the obesity-associated markers in the HFD fed mice.•The effects of NBW on ameliorating obesity were mainly achieved by modulating the gut microbiota in mice. This study aims to investigate the effects of nanobubble water (NBW) on the process of obesity in mice under high-fat diet (HFD). In the experimental groups, HFD feeding mice were supplemented with nitrogen NBW (HFD-N2) or hydrogen NBW (HFD-H2), while the mice fed with standard diet (SD-C) or HFD (HFD-C) supplementation with deionized water were used as control groups. After ten weeks, the concentrations of total cholesterol, alanine aminotransferase and lipopolysaccharide in the mice serum of the HFD-N2 group were significantly lower than those in the HFD-C group. 16S rRNA gene sequencing analysis of the community structures of fecal flora showed that supplementation with N2-NBW to the HFD fed mice could decrease the ratio of Firmicutes to Bacteroidetes. In conclusion, these results demonstrate that supplementation with N2-NBW potentially alleviates the development of obesity in HFD fed mice.