Bioactive Properties of Campomanesia lineatifolia : Correlation Between Anti- Helicobacter pylori Activity, Antioxidant Potential and Chemical Composition

is found in the stomach of patients with chronic gastritis and peptic ulcers, infecting approximately half of the world's population. Current treatment for infection involves a multi-drug therapeutic regime with various adverse effects, which leads to treatment abandonment and contributes to th...

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Veröffentlicht in:Plants (Basel) 2024-11, Vol.13 (22), p.3117
Hauptverfasser: Neves, Nívea Cristina Vieira, de Mello, Morgana Pinheiro, Smith, Sinéad Marian, Boylan, Fabio, Caliari, Marcelo Vidigal, Castilho, Rachel Oliveira
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Sprache:eng
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Zusammenfassung:is found in the stomach of patients with chronic gastritis and peptic ulcers, infecting approximately half of the world's population. Current treatment for infection involves a multi-drug therapeutic regime with various adverse effects, which leads to treatment abandonment and contributes to the emergence of resistant strains of . Previously, we demonstrated that the essential oil of leaves exhibited an anti- activity. In this study, we aimed to evaluate the phenolic content of the phenolic-rich ethanol extract (PEE) from and its anti- and antioxidant properties. Additionally, the anti- activity was assessed in polar and non-polar fractions from PEE, isolated myricitrin (MYR) and a mixture of myricitrin and quercitrin (MYR/QUER) from polar fractions, and aqueous extract (tea) to correlate the responsible fractions or compounds with the observed activity. Broth microdilution assays were performed to assess the anti- activity using type cultures (ATCC 49503, NCTC 11638, both clarithromycin-sensitive) and clinical isolate strains (SSR359, clarithromycin-sensitive, and SSR366, clarithromycin-resistant). The antioxidant activity was evaluated using the DPPH assay. The total tannin and flavonoid contents were determined using the hide-powder method, the Folin-Ciocalteu reagent, and the aluminium chloride colourimetric assay, respectively. The tea (MIC 1:100), PEE, polar and non-polar fractions, MYR, and MYR/QUER inhibited the growth of strains tested (MIC values ranging from 0.49 to 250 μg/mL). The antioxidant assays revealed that PEE exhibited a higher antioxidant activity (EC = 18.47 μg/mL), which correlated to the high phenolic content (tannin and flavonoid, 22.31 and 0.15% / , respectively). These findings support the traditional use of as a multitarget medicinal plant for treating gastric ulcers and reinforce the potential use of the species as a coadjuvant in therapeutic regimes involving patients with resistant infection.
ISSN:2223-7747
2223-7747
DOI:10.3390/plants13223117