Establishment and Comparison of Mouse Pancreatic Cancer Orthotopic Model for Minimally Invasive Ablation Therapy Study

ObjectiveTo explore the advantages and disadvantages of orthotopic mouse model of pancreatic cancer established by orthotopic injection of tumor cells and transplantation of tumor tissue block, in order to provide technical reference for the establishment of orthotopic mouse model of pancreatic cancer with the purpose of precision ablation treatment.MethodsA total of twenty healthy aged 6-8 weeks male C57BL/6 mice were divided into four groups, with five mice per group. Direct injection of mouse pancreatic cancer Panc02 cell was used in group A, direct injection of mouse pancreatic cancer Panc02 cell (containing matrigel) was used in group B, tumor tissue block transplantation of mouse pancreatic cancer was used in group C, the tumor tissue block hydrogel bonding method was used in group D. The tumor formation rate, tumor size, the degree of abdominal organ adhesion, tumor metastasis were observed after surgery in each group of mice. Tumor histomorphology and expressions of Ki67 and α-smooth muscle actin (α-S