A-FABP-PTEN/AKT Regulates Insulin Resistance in Preadipocyte Cell 3T3-L1 Cells

A-FABP-PTEN/AKT Regulates Insulin Resistance in Preadipocyte Cell 3T3-L1 Cells

The purpose of this study was to explore the regulation of A-FABP-PTEN/AKT on insulin resistance in preadipocyte 3T3-L1 cell. siRNA interference method was used to knock-down the A-FABP expression in 3T3-L1 cells. The cell proliferation was detected by oil-O staining and MTT. The protein and mRNA ex...

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Veröffentlicht in:Diabetes, metabolic syndrome and obesity metabolic syndrome and obesity, 2021-01, Vol.14, p.2103-2110
Hauptverfasser: Wu, Rensiqin, Wang, Hui, Huangfu, Jian, Xiao, Rui
Format: Artikel
Sprache:eng
Schlagworte:
a-fabp
Adipocytes
akt
Comparative analysis
Fatty acids
Gene expression
Insulin resistance
Lipids
Metabolism
Obesity
Original Research
Phosphatase
preadipocyte differentiation
Proteins
pten
RNA
Statistical analysis
Variance analysis
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Zusammenfassung:The purpose of this study was to explore the regulation of A-FABP-PTEN/AKT on insulin resistance in preadipocyte 3T3-L1 cell. siRNA interference method was used to knock-down the A-FABP expression in 3T3-L1 cells. The cell proliferation was detected by oil-O staining and MTT. The protein and mRNA expression levels of A-FABP, PTEN and AKT were detected by Western blot and qPCR. Inhibition of A-FABP expression increased cell proliferation activity of the 3T3-L1 cells. Moreover, siRNA3 significantly reduced A-FABP mRNA expression compared with siRNA1 and siRNA2 (
ISSN:1178-7007
1178-7007
DOI:10.2147/DMSO.S305872