Total Phenolic Content and Proliferation Activity of Spirulina Extract in Lymphocyte Cell

Decreased immunity can make the body susceptible to diseases. Many side effects due to the use of drugs such as chemotherapy drugs cause many people to use natural ingredients to be used as supplements. Lymphocyte cell proliferation is cell division or multiplication to enhance the immune system. This study aims to determine the total phenolic content of the 70% ethanolic extract of Spirulina platensis and to analyze the potential of this extract to increase the proliferative activity of lymphocyte cells. The measurement of total phenolic levels used the Folin-Ciocalteu method. The measurement of the viability of lymphocyte cell proliferation activity used the MTT method. The 70% ethanolic extract of S . platensis in this study had a total phenolic content of 2.4957±0.0597 GAE mg/g. The 70% ethanolic extract of S . platensis increased the lymphocyte cell proliferation activity. The best viability results were found at a concentration of 20 ppm of 124.89±1.84%.