Estimation of Flavonoid Content in Propolis by Two Different Colorimetric Methods

Propolis is a natural product with very important properties and its chemical composition was investigated by many researchers. In the present study the polyphenols and flavonoid content was investigated for 10 Romanian propolis samples. Extracts were obtained by using ethanol of different concentrations. The investigation was done spectrophotometrically. Flavonoids content was determined using chrysin as internal standard and zirconium oxychloride as reagent. The second method uses aluminium chloride and 2,4-dinitorphenylhydrazine and as internal standards galangin and pinocembrin.