Evaluation of metabolic changes in liver and serum of streptozotocin-induced diabetic rats after Mango diet supplementation

[Display omitted] •A Mango diet supplementation was performed in STZ-induced diabetic rats.•26 and 29 compounds in serum and liver were annotated as statistically significant.•Metabolites enhanced by the mango diet were associated with the antioxidant system.•The affected pathways by DM were related to fatty acids, bile acids and amino acids. Mango consumption has shown bioactive properties against several diseases like diabetes, therefore, we evaluated how a mango-supplemented diet affects metabolic pathways in diabetic rats. Serum and liver samples were collected from 26 rats divided into 3 groups (healthy, untreated diabetic and mango-treated diabetic) after dietary intervention and analysed using an LC-MS untargeted metabolomic strategy. Twenty-six and 29 metabolites in serum and liver were potentially annotated, showing significant differences among groups. Several affected pathways were due to the disease state [fatty acids (i.e. palmitoleic, linolenic, stearidonic, eicosapentaenoic, docosahexaenoic acids and others), bile acids (i.e. glycocholic acid) and amino acids (i.e. leucine, isoleucine and valine)], and others due to mango supplementation (increased hepatic bioaccumulation of euxanthone, a mangiferin metabolite, and increased glutathione concentration). These results suggest that a mango-supplemented diet exerted a significant antioxidant effect in the liver of diabetic rats, likely due to its phenolic compounds, like mangiferin and its metabolites.