Myco-Suppression Analysis of Soybean ( Glycine max ) Damping-Off Caused by Pythium aphanidermatum

The role of as a biocontrol agent against was investigated to avoid the harmful impacts of fungicides. Three isolates of (MS15, MS19, and MS31) were assessed facing the plant pathogenic the causal agent of damping-off. The tested species were recognized according to their cultural and microscopic characterizations. The identification was confirmed through sequencing of rDNA-ITS regions including the 5.8 S rDNA. The biocontrol agent, , isolates decreased the mycelial growth of the pathogenic with 71.3%, 67.1%, and 68.7% through mycoparasitism on CMA plates. While the half-strength millipore sterilized filtrates of isolates degrade the pathogenic mycelial linear growth by 34.1%, 32.5%, and 31.7%, and reduce the mycelial dry weight of the pathogenic by 40.1%, 37.4%, and 36.8%, respectively. Scanning electron microscopy (SEM) of the most effective antagonistic isolate (MS15) interaction showed coiling, haustorial parts of to hyphae. Furthermore, isolates were proven to enhance the germination of seedling to 93.3% in damping-off infection using agar pots and promote germination of up to 80% during soil pot assay. On the other hand, isolates increase the shoot, root lengths, and the number of lateral roots.