Molecular Characterization of Vancomycin, Mupirocin and Antiseptic Resistant Staphylococcus aureus Strains

is a common cause of nosocomial infections leading to a broad spectrum of diseases. Increasing antibiotic resistance among strains, particularly methicillin-resistant (MRSA), is a serious concern. In addition, the emergence of antiseptics resistance in MRSA helps the organism to persist and spread in healthcare environments easily. The aim of this study was to determine the molecular characteristics of vancomycin, mupirocin, and antiseptic resistant strains. This cross-sectional study was performed on a total of 120 MRSA isolates collected from two major hospitals in Shiraz, Iran. Minimum inhibitory concentrations (MICs) of vancomycin and mupirocin were determined by E-test method according to CLSI and Eucast guidelines. Presence of resistance genes was investigated by PCR method. Antibacterial susceptibility tests for MRSA isolates showed that three isolates (2.5%) were vancomycin-intermediate (VISA), seven isolates (5.8%) were vancomycin-resistant (VRSA), and 15 isolates (12.5%) were high-level mupirocin-resistant (MuH). None of the isolates had vancomycin resistance gene ( ), but the frequency of mupirocin resistance gene was significant, and 55 (45.8%) isolates carried the gene. Moreover, , and genes were detected in 110 (91.7%), 55 (45.8%) and 36 (30%) strains, respectively. This study showed the existence of VISA and VRSA strains in our region, and we also found a high frequency of mupirocin and biocide resistance genes among them.