An Ultrasensitive Biosensor for Detection of Femtogram Levels of the Cancer Antigen AGR2 Using Monoclonal Antibody Modified Screen-Printed Gold Electrodes

The detection of cancer antigens is a major aim of cancer research in order to develop better patient management through early disease detection. Many cancers including prostate, lung, and ovarian secrete a protein disulfide isomerase protein named AGR2 that has been previously detected in urine and...

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Veröffentlicht in:Biosensors (Basel) 2021-06, Vol.11 (6), p.184
Hauptverfasser: Białobrzeska, Wioleta, Dziąbowska, Karolina, Lisowska, Małgorzata, Mohtar, M Aiman, Muller, Petr, Vojtesek, Borivoj, Krejcir, Radovan, O'Neill, Robert, Hupp, Ted R, Malinowska, Natalia, Bięga, Ewelina, Bigus, Daniel, Cebula, Zofia, Pala, Katarzyna, Czaczyk, Elżbieta, Żołędowska, Sabina, Nidzworski, Dawid
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Sprache:eng
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Zusammenfassung:The detection of cancer antigens is a major aim of cancer research in order to develop better patient management through early disease detection. Many cancers including prostate, lung, and ovarian secrete a protein disulfide isomerase protein named AGR2 that has been previously detected in urine and plasma using mass spectrometry. Here we determine whether a previously developed monoclonal antibody targeting AGR2 can be adapted from an indirect two-site ELISA format into a direct detector using solid-phase printed gold electrodes. The screen-printed gold electrode was surface functionalized with the anti-AGR2 specific monoclonal antibody. The interaction of the recombinant AGR2 protein and the anti-AGR2 monoclonal antibody functionalized electrode changed its electrochemical impedance spectra. Nyquist diagrams were obtained after incubation in an increasing concentration of purified AGR2 protein with a range of concentrations from 0.01 fg/mL to 10 fg/mL. In addition, detection of the AGR2 antigen can be achieved from cell lysates in medium or artificial buffer. These data highlight the utility of an AGR2-specific monoclonal antibody that can be functionalized onto a gold printed electrode for a one-step capture and quantitation of the target antigen. These platforms have the potential for supporting methodologies using more complex bodily fluids including plasma and urine for improved cancer diagnostics.
ISSN:2079-6374
2079-6374
DOI:10.3390/bios11060184