Plant regeneration in Curcuma species and assessment of genetic stability of regenerated plants

An efficient plant regeneration protocol was developed from rhizomes of two Curcuma species, C. longa and C. amada. Response was highly dependent on the season, with above 69 % of culture developing adventitious shoots during spring. Greatest regeneration and multiplication was observed in modified...

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Veröffentlicht in:	Biologia plantarum 2010-09, Vol.54 (3), p.423-429
Hauptverfasser:	Das, A.,Indian Inst. of Technology, Guwahati (India). Dept. of Biotechnology, Kesari, V.,Indian Inst. of Technology, Guwahati (India). Dept. of Biotechnology, Rangan, L.,Indian Inst. of Technology, Guwahati (India). Dept. of Biotechnology
Format:	Artikel
Sprache:	eng
Schlagworte:	6-BENZILADENINA 6-BENZYLADENINE AIB ANA axillary bud AZUCARES Biomedical and Life Sciences BROTES CULTIVO IN VITRO CULTURE IN VITRO CULTURE MEDIA CURCUMA AMADA CURCUMA LONGA ELECTROFORESIS ELECTROPHORESE ELECTROPHORESIS GENETIC VARIATION http://www.fao.org/aos/agrovoc#c_10204 http://www.fao.org/aos/agrovoc#c_14261 http://www.fao.org/aos/agrovoc#c_15975 http://www.fao.org/aos/agrovoc#c_2030 http://www.fao.org/aos/agrovoc#c_2034 http://www.fao.org/aos/agrovoc#c_24135 http://www.fao.org/aos/agrovoc#c_24136 http://www.fao.org/aos/agrovoc#c_24894 http://www.fao.org/aos/agrovoc#c_2523 http://www.fao.org/aos/agrovoc#c_34114 http://www.fao.org/aos/agrovoc#c_36341 http://www.fao.org/aos/agrovoc#c_3780 http://www.fao.org/aos/agrovoc#c_4102 http://www.fao.org/aos/agrovoc#c_4260 http://www.fao.org/aos/agrovoc#c_4550 http://www.fao.org/aos/agrovoc#c_5061 http://www.fao.org/aos/agrovoc#c_5691 http://www.fao.org/aos/agrovoc#c_5968 http://www.fao.org/aos/agrovoc#c_5977 http://www.fao.org/aos/agrovoc#c_6565 http://www.fao.org/aos/agrovoc#c_6651 http://www.fao.org/aos/agrovoc#c_7507 http://www.fao.org/aos/agrovoc#c_7538 http://www.fao.org/aos/agrovoc#c_754 IBA IN VITRO CULTURE IN VITRO REGENERATION KINETIN KINETINE LARGURA LENGTH Life Sciences LONGUEUR MALTOSA MALTOSE MEDIO DE CULTIVO MICROPROPAGACION MICROPROPAGATION MILIEU DE CULTURE MULTIPLICATION DES PLANTES NAA Original Papers PEPTIDE PEPTIDES PEPTIDOS PLANT GROWTH SUBSTANCES PLANT PROPAGATION Plant Sciences POUSSE PROPAGACION DE PLANTAS QUINETINA RACINE RAICES RAPD REGENERACION IN VITRO REGENERATION IN VITRO RHIZOME RHIZOMES RIZOMAS ROOTS sds-page SEASONAL VARIATION SHOOTS SUBSTANCE DE CROISSANCE VEGETALE SUCRES SUGARS SUPERVIVENCIA SURVIE SURVIVAL SUSTANCIAS DE CRECIMIENTO VEGETAL VARIACION ESTACIONAL VARIACION GENETICA VARIATION GENETIQUE VARIATION SAISONNIERE zingiberaceae
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Zusammenfassung:	An efficient plant regeneration protocol was developed from rhizomes of two Curcuma species, C. longa and C. amada. Response was highly dependent on the season, with above 69 % of culture developing adventitious shoots during spring. Greatest regeneration and multiplication was observed in modified Murashige and Skoog (MS) medium supplemented with 13.31 microM benzyladenine and 2.68 microM alpha-naphthalene acetic acid (NAA) in C. longa or 2.46 microM indolebutyric acid in C. amada. Effect of sugars and agar at different concentrations were also studied and 2 % maltose and 0.7 % agar were found optimum for shoot multiplication and regeneration. Most plantlets developed roots simultaneously but others formed roots when subcultured in 1/2 MS medium supplemented with 2.68 microM NAA. Plants were successfully hardened in greenhouse with 80 % survival. The genetic purity of micropropagated plantlets was analyzed using RAPD and protein profiles.
ISSN:	0006-3134 1573-8264
DOI:	10.1007/s10535-010-0077-0