Early detection of oral bacteria causing gum infections and dental caries in children

Periodontal diseases are resulted from gum infections and dental plaques, which are mainly caused by the bacterial agents. Since dental monitoring includes important prognostic roles, the aim of this study was to detect the most common periodontal pathogenic bacteria in children. A total of 200 clinical samples were collected from dental plaques and gingival grooves. Target-specific primers were designed for in , in and 16S rRNA in and . Then, a multiplex polymerase chain reaction method was optimized for the highlighted bacterial agents. In general, the highest and the lowest bacterial prevalence rates belonged to (88%) and (13%), respectively. Furthermore, prevalence rates of , and were 25, 21 and 45% in samples, respectively. There were significant associations between dental/oral health and microbial community. Metabolism of the oral bacteria, including biofilm formation, can affect gums and develop dental plaques and hence dental caries, especially in children. Early diagnosis of dental caries in children via rapid, accurate molecular methods can increase the diagnostic capacity in clinical cases and therefore prevent periodontal infections in adulthood.