Snai2‐mediated upregulation of NADSYN1 promotes bladder cancer progression by interacting with PHB

Upon intersecting three distinct gene sets (three groups of differential gene between MIBC and NMIBC), we observed upregulation of PHB in MIBC tissues (Figures 1A and S1). The peak distributions of tumour samples tended to be enriched in the middle of the gene body, whereas the PHB binding peaks tended to be enriched near the transcription start site in the proximal part of the gene (Figures S2D and S2E). TGF-β increased TGFβR-1/ALK5 inhibitor RepSox decreased NADSYN1 expression (Figure S11D).7 Immunohistochemical analysis of Snai2, NADSYN1, PHB, CDH1, and CDH2 demonstrated that in tissues with NADSYN1 high expression, the level of PHB, Snai2 and CDH2 was also high and the level of CDH1 was low (Figure S10D). [...]low Snai2 and NADSYN1 expression was associated with significantly longer survival of bladder cancer patients (Figure S11E).