Monoclonal antibody against Rab8 from Bombyx mori (Lepidoptera: Bombycidae)

Small GTPases of the Rab family are key regulators of membrane trafficking. Monoclonal antibodies are useful tools for identifying proteins that interact with other proteins and for examining their tissue distribution. We selected a monoclonal antibody against Rab8 of Bombyx mori L. It specifically...

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Veröffentlicht in:	European journal of entomology 2007-10, Vol.104 (4), p.641-645
Hauptverfasser:	Uno, T.,Kobe Univ. (Japan). Dept. of Biofunctional Chemistry, Nakada, T.,Kobe Univ. (Japan). Dept. of Biofunctional Chemistry, Uno, Y.,Kobe Univ. (Japan). Dept. of Plant Resource Science, Kanamaru, K.,Kobe Univ. (Japan). Dept. of Biofunctional Chemistry, Yamagata, H.,Kobe Univ. (Japan). Dept. of Biofunctional Chemistry, Nakamura, M.,Kyoto Gakuen Univ. (Japan). Dept. of Bioscience and Biotechnology, Takagi, M.,Kobe Univ. (Japan). Dept. of Animal Science
Format:	Artikel
Sprache:	eng
Schlagworte:	ANTICORPS MONOCLONAL ANTICUERPOS MONOCLONALES Bombycidae BOMBYX MORI brain Butterflies & moths ELECTROFORESIS ELECTROPHORESE ELECTROPHORESIS ELISA Entomology http://www.fao.org/aos/agrovoc#c_15968 http://www.fao.org/aos/agrovoc#c_24051 http://www.fao.org/aos/agrovoc#c_2523 http://www.fao.org/aos/agrovoc#c_32817 http://www.fao.org/aos/agrovoc#c_6259 http://www.fao.org/aos/agrovoc#c_9357 IMMUNOBLOTTING Kinases Lepidoptera MONOCLONAL ANTIBODIES monoclonal antibody PROTEINAS PROTEINE PROTEINS rab proteins TEST ELISA
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Zusammenfassung:	Small GTPases of the Rab family are key regulators of membrane trafficking. Monoclonal antibodies are useful tools for identifying proteins that interact with other proteins and for examining their tissue distribution. We selected a monoclonal antibody against Rab8 of Bombyx mori L. It specifically recognized amino acid residues 30-109, which are conserved among Rab8 proteins, and did not recognize any other Rab proteins. Western blotting using the antibody revealed one band in the brains of B. mori and rat. Far-Western blotting analysis detected three proteins interacting with Rab8. These results indicate that this antibody is useful for clarifying the physiological function of Rab8 of B. mori and other species. This is a report of a study on a monoclonal antibody against insect Rab protein.
ISSN:	1210-5759 1802-8829
DOI:	10.14411/eje.2007.080