Fluorescence microscope light source based on integrated LED

An LED-integrated excitation cube (LEC) was designed to address the limitations of conventional fluorescence lamps. The LEC has a decentralized structure, high optical power density, and efficient illumination. The optical efficiency of LECs is 1–2 orders of magnitude higher than that of mercury lamps, enabling high-quality fluorescence imaging with spectral coverage from UV to red. LECs can be easily installed on commercial fluorescence microscopes by replacing conventional fluorescence filter cubes, and a built-in LEC driver can identify the types of LEDs in different spectral bands to adopt optimal operating conditions. The LED-integrated excitation cube (LEC) is compact, low-cost, and easy to install, taking full advantage of the benefits of LED excitations, and can be used in any fluorescent microscope.