Brassinosteroid-induced transcriptomic rearrangements unveiled the physiological mechanism of chromium stress tolerance in Brassica napus

Brassinosteroid (BR), a plant hormone regulating growth, development, and stress responses, emerges as a promising tool for maintaining agricultural production under abiotic stress conditions. In this study, we conducted RNA-seq profiling and morpho-physiological analysis to investigate the molecula...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Current plant biology 2024-09, Vol.39, p.100360, Article 100360
Hauptverfasser: Wu, Xiaofen, Li, Lan, Hannan, Fakhir, Qin, Tongjun, Ayyaz, Ahsan, Ma, Jiali, Athar, Habib Ur Rehman, Zafar, Zafar Ullah, Farooq, Muhammad Ahsan, Zhou, Weijun
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Brassinosteroid (BR), a plant hormone regulating growth, development, and stress responses, emerges as a promising tool for maintaining agricultural production under abiotic stress conditions. In this study, we conducted RNA-seq profiling and morpho-physiological analysis to investigate the molecular cross-talk involved in 24-epibrassinolide (EBR) mediating alleviation of chromium (Cr) stress. EBR inhibited Cr accumulation and reversed Cr-induced phytotoxicity, thereby promoting plant growth. The photosynthetic pigments, chlorophyll fluorescence a, electron transport rate (ETR) and non-photochemical quenching (NPQ) were significantly higher in EBR+Cr treated plants compared to Cr alone. EBR application facilitated the recovery from Cr-induced structural deformities, including the disintegration of cell walls and membranes. Furthermore, under Cr stress, EBR application reduced malondialdehyde (MDA) and reactive oxygen species (ROS) production and accumulation. The levels of glutathione reductase (GR) and the activities of antioxidant enzymes were notably higher in plants subjected to EBR application following Cr stress. In addition, we established a transcriptomic database comprising 2345 differentially expressed genes (DEGs) (1255 upregulated and 1090 downregulated) as a result of EBR application under Cr stress. The transcriptome analysis unveiled key DEGs and the associated pathways, emphasizing the importance of defense responses, genes encoding photosystem I and II, jasmonate signaling, aquaporins, ABC transporters, and cell wall biogenesis-related genes in the response of EBR to Cr stress. •Cr stress inhibits the plant growth and physiological attributes of B. napus L. plants.•The application of EBR significantly reduces the toxic effects of Cr in B. napus plants.•EBR treatment improves photosynthesis, decreases oxidative stress, and reduces Cr levels.•EBR application regulates defense related genes and protects ultra-cellular structures.•Transcriptomic data revealed DEGs and enriched pathways associated with EBR response to Cr stress.
ISSN:2214-6628
2214-6628
DOI:10.1016/j.cpb.2024.100360