Untargeted metabonomic analysis of a cerebral stroke model in rats: a study based on UPLC–MS/MS

Brain tissue damage caused by ischemic stroke can trigger changes in the body's metabolic response, and understanding the changes in the metabolic response of the gut after stroke can contribute to research on poststroke brain function recovery. Despite the increase in international research on poststroke metabolic mechanisms and availability of powerful research tools in recent years, there is still an urgent need for poststroke metabolic studies. Metabolomic examination of feces from a cerebral ischemia-reperfusion rat model can provide new insights into poststroke metabolism and identify key metabolic pathways, which will help reveal diagnostic and therapeutic targets as well as inspire pathophysiological studies after stroke. We randomly divided 16 healthy adult pathogen-free male Sprague-Dawley (SD) rats into the normal and the study group after middle cerebral artery occlusion/reperfusion(MCAO/R). After 1 and 14 days of modelling, compared to the study group, rats in the study group showed significant neurological deficits (P