Differentially expressed homologous genes reveal interspecies differences of Paragonimus proliferus based on transcriptome analysis

( ), one of 46 species registered in the National Center for Biotechnology Information database, may be much more widely distributed in Southeast Asia than previously thought, as its reported natural foci have increased in the past decades. However, very little is known about its molecular biology, especially at the transcriptome level. For the first time, the transcriptome of this species was sequenced and compared with four other common species, namely , , , and , to predict homologous genes and differentially expressed homologous genes to explore interspecies differences of A total of 7393 genes were found to be significantly differentially expressed. Of these, 49 were considered to be core genes because they were differentially expressed in all four comparison groups. Annotations revealed that these genes were related mainly to “duplication, transcription, or translation”, energy or nutrient metabolism, and parasitic growth, proliferation, motility, invasion, adaptation to the host, or virulence. Interestingly, a majority (5601/7393) of the identified genes, and in particular the core genes (48/49), were expressed at lower levels in . The identified genes may play essential roles in the biological differences between species. This work provides fundamental background information for further research into the molecular biology of