DNA Optical Detection Based on Porous Silicon Technology: from Biosensors to Biochips

DNA Optical Detection Based on Porous Silicon Technology: from Biosensors to Biochips

A photochemical functionalization process which passivates the porous silicon surface of optical biosensors has been optimized as a function of the thickness and the porosity of the devices. The surface modification has been characterized by contact angle measurements. Fluorescence measurements have...

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Veröffentlicht in:Sensors (Basel, Switzerland) Switzerland), 2007-02, Vol.7 (2), p.214-221
Hauptverfasser: De Stefano, Luca, Arcari, Paolo, Lamberti, Annalisa, Sanges, Carmen, Rotiroti, Lucia, Rea, Ilaria, Rendina, Ivo
Format: Artikel
Sprache:eng
Schlagworte:
Biochemistry
Biochip
Biosensors
Contact angle
Full Research Paper
Nanostructured materials
Optical Biosensors
Porous Silicon
Sensors
Silicon
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Zusammenfassung:A photochemical functionalization process which passivates the porous silicon surface of optical biosensors has been optimized as a function of the thickness and the porosity of the devices. The surface modification has been characterized by contact angle measurements. Fluorescence measurements have been used to investigate the stability of the DNA single strands bound to the nanostructured material. A dose-response curve for an optical label-free biosensor in the 6-80 μM range has been realized.
ISSN:1424-8220
1424-8220
DOI:10.3390/s7020214