Diaskintest for the diagnosis of bovine tuberculosis

The objective of the study : to compare the diagnostic value of intracutaneous tests with tuberculin and diaskintest for detection of tuberculosis in cattle. Subjects and methods . In this study, PPD tuberculin for mammals was used, it was made from M. bovis strain AN-5, manufactured by BIOK, and Diaskintest (recombinant tuberculous allergen, solution forintracutaneous administration) manufactured by ZAO FF Lecco, series 030307. Laboratory animals (guinea pigs, rabbits, chickens) and farm animals (pigs and cattle) were used in the study. Results . It has been established on laboratory animals that diaskintest does not cause any adverse events. When assessing sows sensitized with M. avium , it was found that the intracutaneous administration of PPD tuberculin to mammals resulted in up to 8.5% of positive reactions, while diaskintest was negative in all of them. Among cattle sensitized to non-tuberculous mycobacteria, intracutaneous administration of tuberculin for mammals revealed up to 4.6% of positive reactions, and diaskintest – up to 0.6%. In the farm with the unfavorable TB situation where animals infected with M. bovis were previously detected, when examining 177 cows, tuberculin test was positive in 102 (57.6%) of them, and diaskintest in 88 (49.7%). Diaskintest can be used for differential diagnosis of tuberculosis and sensitization by non-tuberculous mycobacteria in pigs and cattle.