Study on changes of polyamine levels in mice with the development of U14 cervical cancer

This study was performed to investigate the possible involvement of polyamincs in the development of cervical cancer. The objective of the present study was therefore to lind the specilic polyamine indicators, which could be used as useful markers for the early determination of cervical cancer. A simple method for the simultaneous determination of plasma concentrations ol live polyamines in normal and UI4 model mice was developed by using HPLC-MS. The samples were derivatized by benzoyl chloride. The derived polyamines were separated on a C18 colunm by a gradient elution with methanol water, and then detected with HPLC-MS. The results showed tha! all polyamine levels in the U 14 model mice were higher than those in normal ones. Thc cadaverine. putrescine and 1,3-diaminopropane levels were significantly higher in U 14 model mice plasma than those in normal mice plasma, especially the putrescine and 1, 3-diaminopropane （P〈0.01）. The cadaverine, putrescine and 1, 3-diaminopropane levels were significantly higher in U 14 mice model urine than those in normal mice urine, especially the cadaverine and 1, 3-diaminopropane （P〈0.01）. Putrescine, cadaverine and I, 3-diaminopropane might be the indicators of the cervical cancer.