Effects of the Monomeric Components of Poly-hydroxybutyrate-co-hydroxyhexanoate on the Growth of Vibrio penaeicida In Vitro and on the Survival of Infected Kuruma Shrimp ( Marsupenaeus japonicus )

Here, we investigated the inhibitory effects of the biodegradable, water-insoluble polymer poly-hydroxybutyrate-co-hydroxyhexanoate (PHBH) and its two constituent monomers, the hydroxyalkanoic acids 3-hydroxybutyrate (3HB) and 3-hydroxyhexanoate (3HH), on the growth of the shrimp-pathogenic bacteriu...

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Veröffentlicht in:Animals (Basel) 2021-02, Vol.11 (2), p.567
Hauptverfasser: Fukami, Kimio, Takagi, Fumika, Sonoda, Kohei, Okamoto, Hiroshi, Kaneno, Daisuke, Horikawa, Takao, Takita, Masaki
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Sprache:eng
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Zusammenfassung:Here, we investigated the inhibitory effects of the biodegradable, water-insoluble polymer poly-hydroxybutyrate-co-hydroxyhexanoate (PHBH) and its two constituent monomers, the hydroxyalkanoic acids 3-hydroxybutyrate (3HB) and 3-hydroxyhexanoate (3HH), on the growth of the shrimp-pathogenic bacterium . In vitro experiments revealed that 3HH showed greater growth inhibitory activity than 3HB against . In addition, the activities of hydroxyalkanoic acids were pH dependent, being greater at pH 6.0 than at pH 7.0. Investigation of the pH of the shrimp gut revealed a pH range of 5.9-6.7 (mean 6.29 ± SD 0.20), indicating that the physiological environment was suitable for 3HB and 3HH to exert their inhibitory activities against . In vivo bacterial challenge experiments revealed that survival rates in kuruma shrimp ( ) infected by were significantly increased in shrimp reared on feed containing PHBH (0.1%-5% / PHBH) compared with that in shrimp reared on standard diet alone. Supplementation with PHBH had no significant effects on three shrimp growth parameters: increase in body weight, daily feeding rate, and feed conversion ratio. These results suggest that supplementation of standard diet with PHBH will increase shrimp resistance to infection by , thereby increasing shrimp aquaculture productivity.
ISSN:2076-2615
2076-2615
DOI:10.3390/ani11020567