Targeted amplification-based whole genome sequencing of Monkeypox virus in clinical specimens

Targeted amplification-based whole genome sequencing of Monkeypox virus in clinical specimens

We present a protocol to efficiently sequence genomes of the MPXV-causing mpox. This enables researchers and public health agencies to acquire high-quality genomic data using a rapid and cost-effective approach. Genomic data can be used to conduct surveillance and investigate mpox outbreaks. We pres...

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Veröffentlicht in:Microbiology spectrum 2024-01, Vol.12 (1), p.e0297923-e0297923
Hauptverfasser: Isabel, S, Eshaghi, A, Duvvuri, V R, Gubbay, J B, Cronin, K, Li, Aimin, Hasso, M, Clark, S T, Hopkins, J P, Patel, S N, Braukmann, T W A
Format: Artikel
Sprache:eng
Schlagworte:
Clinical Microbiology
Disease Outbreaks
Genomics
Humans
Methods and Protocols
Monkeypox virus
Monkeypox virus - genetics
mpox
Mpox (monkeypox)
Ontario - epidemiology
targeted amplification
WGS
Whole Genome Sequencing
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Zusammenfassung:We present a protocol to efficiently sequence genomes of the MPXV-causing mpox. This enables researchers and public health agencies to acquire high-quality genomic data using a rapid and cost-effective approach. Genomic data can be used to conduct surveillance and investigate mpox outbreaks. We present 91 mpox genomes that show the diversity of the 2022 mpox outbreak in Ontario, Canada.
ISSN:2165-0497
2165-0497
DOI:10.1128/spectrum.02979-23