Generation of an NCS1 gene knockout human induced pluripotent stem cell line using CRISPR/Cas9

NCS1 (Neuronal calcium sensor protein 1) encodes a highly conserved calcium binding protein abundantly expressed in neurons. It modulates intracellular calcium homeostasis, calcium-dependent signaling pathways as well as neuronal transmission and plasticity. Here, we generated a NCS1 knockout human...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Stem cell research 2023-12, Vol.73, p.103253-103253, Article 103253
Hauptverfasser:	Maierhof, Smilla K, Schinke, Christian, Cernoch, Janine, Hew, Lois, Bruske, Laura Pauline, Fernandez Vallone, Valeria, Fischer, Kristin, Stachelscheid, Harald, Huehnchen, Petra, Endres, Matthias, Diecke, Sebastian, Telugu, Narasimha Swamy, Boehmerle, Wolfgang
Format:	Artikel
Sprache:	eng
Schlagworte:	Calcium - metabolism CRISPR-Cas Systems - genetics Gene Editing Gene Knockout Techniques Humans Induced Pluripotent Stem Cells - metabolism
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	NCS1 (Neuronal calcium sensor protein 1) encodes a highly conserved calcium binding protein abundantly expressed in neurons. It modulates intracellular calcium homeostasis, calcium-dependent signaling pathways as well as neuronal transmission and plasticity. Here, we generated a NCS1 knockout human induced pluripotent stem cell (hiPSC) line using CRISPR-Cas9 genome editing. It shows regular expression of pluripotent markers, normal iPSC morphology and karyotype as well as no detectable off-target effects on top 6 potentially affected genes. This newly generated cell line constitutes a valuable tool for studying the role of NCS1 in the pathophysiology of various neuropsychiatric disorders and non-neurological disease.
ISSN:	1873-5061 1876-7753
DOI:	10.1016/j.scr.2023.103253