Comparative study of heparin-binding proteins profile of Murrah buffalo (Bubalus bubalis) semen

Aim: The experiment was conducted to study the total seminal plasma protein (TSPP) and heparin-binding proteins (HBPs) in relation to initial semen quality of buffalo bull. Materials and Methods: Semen from two Murrah buffalo bulls (bull no. 605 and 790) with mass motility of ≥ 3+ were used for the study and categorized into three groups (Group I- Mass motility 3+, Group II- Mass motility 4+ and Group III- Mass motility 5+). Seminal plasma from semen was separated by centrifugation. HBPs was isolated and purified from heparin-agarose affinity column by modified elution buffer. TSPP and isolated HBPs concentration was estimated by Lowry's method. The purified HBPs were resolved on Sodium dodecyl sulfate polyacrylamide gel electrophoresis to check the protein profile of two bulls. Results: The mean values of TSPP concentrations in bull no. 605 and 790 in Group I, II and III were 30.64 ± 0.12, 31.66 ± 0.09, 32.53 ± 0.19 and 28.51 ± 0.09, 29.49 ± 0.15, 30.45 [+ or -] 0.17 mg/mL, respectively. The mean values of HBPs concentrations in bull no. 605 and 790 in Group I, II and III were 3.11 ± 0.07, 3.32 ± 0.06, 3.46 [+ or - ] 0.08 and 2.51 ± 0.08, 2.91 ± 0.05, 3.10 [+ or -] 0.03 mg/mL, respectively. Both the values of TSPP and HBPs were significantly higher (p