Therapeutic Effect of Recombinant Salmonella VNP20009-M on Osteosarcoma

Objective To investigate the therapeutic effect of attenuated salmonella VNP20009-M that carries a methioninase gene on osteosarcoma. Methods Recombinant salmonella VNP20009-M was co-cultured with a human osteosarcoma cell line. After the methioninase gene was overexpressed in osteosarcoma cells, cell proliferation, migration and apoptosis were detected. Finally, we evaluated the effect of the attenuated salmonella VNP20009-M on the subcutaneous xenograft in the nude mice by intratumorally injecting different dosages of VNP20009-M. Results It was verified by plasmid PCR that the methioninase gene was only found in the target strain VNP20009-M and had high methioninase activity. The recombinant salmonella was successfully constructed. VNP20009-M significantly induced the apoptosis of MNNGHOS cells. Overexpression of the L-methioninase gene in the osteosarcoma cells significantly inhibited cell proliferation and migration, compared with the control group. The body weight of the subcutaneous tumorbearing mice af