Regulatory mechanisms of lipopolysaccharide synthesis in Escherichia coli
Lipopolysaccharide (LPS) is an essential glycolipid and forms a protective permeability barrier for most Gram-negative bacteria. In E. coli , LPS levels are under feedback control, achieved by FtsH-mediated degradation of LpxC, which catalyzes the first committed step in LPS synthesis. FtsH is a mem...
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Veröffentlicht in: | Nature communications 2022-08, Vol.13 (1), p.4576-4576, Article 4576 |
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Sprache: | eng |
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Zusammenfassung: | Lipopolysaccharide (LPS) is an essential glycolipid and forms a protective permeability barrier for most Gram-negative bacteria. In
E. coli
, LPS levels are under feedback control, achieved by FtsH-mediated degradation of LpxC, which catalyzes the first committed step in LPS synthesis. FtsH is a membrane-bound AAA+ protease, and its protease activity toward LpxC is regulated by essential membrane proteins LapB and YejM. However, the regulatory mechanisms are elusive. We establish an in vitro assay to analyze the kinetics of LpxC degradation and demonstrate that LapB is an adaptor protein that utilizes its transmembrane helix to interact with FtsH and its cytoplasmic domains to recruit LpxC. Our YejM/LapB complex structure reveals that YejM is an anti-adaptor protein, competing with FtsH for LapB to inhibit LpxC degradation. Structural analysis unravels that LapB and LPS have overlapping binding sites in YejM. Thus, LPS levels control formation of the YejM/LapB complex to determine LpxC protein levels.
Synthesis of lipopolysaccharides, essential components for most gram-negative bacteria, is under tight control. Here, authors use in vitro reconstitution and structural approaches to elucidate some of these regulatory mechanisms involving essential membrane proteins LapB and YejM. |
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ISSN: | 2041-1723 2041-1723 |
DOI: | 10.1038/s41467-022-32277-1 |