Genome-wide identification and expression analysis of GDP-D-mannose pyrophosphorylase and KATANIN in Corymbia citriodora

The GDP-D-mannose pyrophosphorylase ( ) and microtubule severing enzyme KATANIN ( ) are crucial for wood formation. Although functional identification has been performed in , few comprehensive studies have been conducted in forest trees. In this study, we discovered 8 and 4 genes by analyzing the whole genome sequence of . The chromosomal location, genome synteny, phylogenetic relationship, protein domain, motif identification, gene structure, cis-acting regulatory elements, and protein-interaction of and were all investigated. has just one pair of segmentally duplicated genes, while has no duplication events. According to gene structure, two 5' UTRs were identified in . Furthermore, there is no protein-interaction between KTN and GMP. Based on real-time PCR, the expression of most genes showed a positive connection with DBH diameters. In addition, the expression of and genes were greater in different size tree, indicating that these genes are important in secondary xylem production. Overall, this findings will enhance our comprehension of the intricacy of & across diverse DBHs and furnish valuable insights for future functional characterization of specific genes in