Multiplication of Acer davidii ssp. grosseri (Pax) De Jong under in vitro conditions

Experimental works were focused on creation of optimal protocol for Acer davidii ssp. grosseri shoots multiplication. As the basic medium we used MS (Murashige α Skoog) culture medium enriched by cytokinins TDZ (Thidiazuron) and BAP (6-Benzylaminopurine) in different concentrations. There were used single nodal segments from four years old mother plants in experiments. Experiments were established in the middle of June in the phase of rapid growth of shoots. Cultivation conditions were: 21°C, light intensity 40 μmol . m−2. s−1, period of light 16 hours during the day. The reactions of explants were evaluated after six and twelve month. The results were statistically analysed. The explants of Acer davidii ssp. grosseri reacted differently on hormonal regulation. The lowest multiplication activity of explants was found out on MS culture medium enriched by 2,6 μM BAP. The average shoot multiplication on MS + 0,5 μM TDZ culture medium was 1,2. Multiplication 1,49 shoot per explants was found out on MS + 2,6 μM BAP + 0,5 μM TDZ. On the basic MS medium 1,35 shoots per explants multiplicated. In spite of positive influence of application of TDZ and BAP on shoot multiplication the results compared with multiplication on MS medium were statistically insignificant.