An isothermal CRISPR-based diagnostic assay for Neisseria gonorrhoeae and Chlamydia trachomatis detection

A method for (NG)/ (CT) detection is developed using multiplex-recombinase polymerase amplification and Cas12a/Cas13a. This method can detect NG and CT simultaneously with high sensitivity and specificity. This method has great potential to be further developed into larger-scale screening and point-...

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Veröffentlicht in:	Microbiology spectrum 2023-12, Vol.11 (6), p.e0046423-e0046423
Hauptverfasser:	Luo, Hao, Zeng, Lihong, Yin, Xiaona, Pan, Yuying, Yang, Jianjiang, Liu, Mingjing, Qin, Xiaolin, Feng, Zhanqin, Chen, Wentao, Zheng, Heping
Format:	Artikel
Sprache:	eng
Schlagworte:	Chlamydia Infections - diagnosis Chlamydia trachomatis Chlamydia trachomatis - genetics Clinical Microbiology coinfection CRISPR‒Cas12a CRISPR‒Cas13a dual-target detection Gonorrhea - diagnosis Humans Neisseria gonorrhoeae Neisseria gonorrhoeae - genetics Research Article Sensitivity and Specificity
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Beschreibung
Zusammenfassung:	A method for (NG)/ (CT) detection is developed using multiplex-recombinase polymerase amplification and Cas12a/Cas13a. This method can detect NG and CT simultaneously with high sensitivity and specificity. This method has great potential to be further developed into larger-scale screening and point-of-care testing (POCT).
ISSN:	2165-0497 2165-0497
DOI:	10.1128/spectrum.00464-23