Ischemic postconditioning of the isolated human myocardium: Role of the applied protocol

Ischemic postconditioning (IPostC), has been proposed as a useful approach to reduce infarct size in all species, but its clinical utility remains unclear. To investigate the role played by the protocol used on the efficacy of IPostC in protecting the diseased human myocardium. Myocardial atrial sam...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:International journal of cardiology. Heart & vasculature 2015-09, Vol.8 (C), p.55-61
Hauptverfasser: Casós, Kelly, Pérez, María-Llanos, Blasco-Lucas, Arnau, Ferrer-Curriu, Gemma, Gracia-Baena, Juan Manuel, Sureda, Carlos, Permanyer, Eduard, Igual, Alberto, Galiñanes, Manuel
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Ischemic postconditioning (IPostC), has been proposed as a useful approach to reduce infarct size in all species, but its clinical utility remains unclear. To investigate the role played by the protocol used on the efficacy of IPostC in protecting the diseased human myocardium. Myocardial atrial samples from patients were subjected to a 90min ischemia/120min reoxygenation followed by different IPostC protocols to investigate the role of the time of ischemia (30, 60, 90 and 120s) and the number of cycles (1, 2, 3 and 4) with 60 and 120s of total ischemic time. Muscles were also subjected to ischemic preconditioning (IPreC). The release of lactate dehydrogenase (LDH) and the measurement of tetrazolium bromide (MTT) were determined. IPostC increased the LDH and decreased the MTT values from those of control, independently of the duration of the conditioning ischemia. LDH and MTT values also worsened by augmenting the number of IPostC cycles whereas they were significantly improved by IPreC. However, analysis of individual results indicated that in approximately 1/3 of the cases IPostC exhibited some degree of protection especially in the presence of increased ischemic injury. The present findings show that IPostC of the human myocardium may be influenced by the protocol used and also by the degree of the preceding ischemic injury. IPostC was beneficial in approximately 1/3 of the cases; however in the remaining cases it increased ischemic damage and, therefore, these results raise a word of caution on its broad clinical use.
ISSN:2352-9067
2352-9067
DOI:10.1016/j.ijcha.2015.05.009