Micropropagation of Acacia chundra (Roxb.) DC

An in vitro propagation of an economic leguminous tree, Acacia chundra, has been standardized. Induction of bud sprout was obtained from shoot tip and nodal explants derived from in vitro grown plants of A. chundra on the Murashige and Skoog (MS) basal medium supplemented with 6-benzylaminopurine (BA, 1.0 mg/L) and 20 mg/L adenine sulfate (Ads). The rate of multiplication was obtained on MS medium supplemented with 1.5 mg/L BA, 0.01 to 0.05 mg/L indole-3-acetic acid (IAA) and 50 mg/L Ads. The multiplication rate varied from 3 to 6 shoots depending on the growth regulators used. Excised shoots were rooted on half-strength MS basal salts supplemented with 0.25 mg/L indole-3-butyric acid or IAA and 20 g/L (w/v) sucrose after 10 to 12 days of culture. The micropropagated plantlets were acclimatized and successfully transferred to soil.