Development and optimization of bioanalytical parameters for the standardization of Trigonella foenum–graecum

To develop a novel qualitative and quantitative method, which can pave the way for rapid and selective determination of different constituents of Trigonella foenum–graecum (T. foenum–graecum). Phytochemical analysis, solubility test, heavy metal analysis, antimicrobial study and quantitative analysis through HPTLC techniques were performed in the present investigation. Phytochemical analysis showed to contain alkaloid, saponin, tannin, flavonoid, steroid, triterpenoid, glycoside and protein. pH of the 1% solution was found to be 5.65, loss on drying was 4.5%, total ash, solubility in water and in 50% alcohol was 7.11%, 81.00% and 84.25% respectively. Total flavonoid and total phenol content were found to be 1.8% and 0.60% (w/w). Level of different heavy metals and microorganisms were found to be under the limit. Content of quercetin and rutin in the T. foenum–graecum were found to be 0.879% (w/w) and 0.417% (w/w) respectively. In the future, these phytochemical parameters could be used as an important tool for the standardization of T. foenum–graecum.