Generation of a homozygous ABCA7-knockout human iPSC line using the CRISPR/Cas9 system

Generation of a homozygous ABCA7-knockout human iPSC line using the CRISPR/Cas9 system

ABCA7 encodes an ATP-binding cassette transporter, and its loss-of-function variants are associated with Alzheimer’s disease. To investigate the role of ABCA7 deficiency in the pathogenesis of Alzheimer’s disease, we generated a homozygous ABCA7-knockout induced pluripotent stem cell (iPSC) line usi...

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Veröffentlicht in:Stem cell research 2023-02, Vol.66, p.103000-103000, Article 103000
Hauptverfasser: Guan, Mingfeng, Chai, Yue, Yi, Ran, Chen, Yuewen, Ip, Jacque P.K., Ye, Tao, Chen, Yu
Format: Artikel
Sprache:eng
Schlagworte:
Alzheimer Disease - genetics
ATP-Binding Cassette Transporters - genetics
CRISPR-Cas Systems - genetics
Homozygote
Humans
Induced Pluripotent Stem Cells - metabolism
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Zusammenfassung:ABCA7 encodes an ATP-binding cassette transporter, and its loss-of-function variants are associated with Alzheimer’s disease. To investigate the role of ABCA7 deficiency in the pathogenesis of Alzheimer’s disease, we generated a homozygous ABCA7-knockout induced pluripotent stem cell (iPSC) line using CRISPR/Cas9-mediated gene editing. This ABCA7-deficient iPSC line maintains a normal karyotype, expression of pluripotency markers, and trilineage differentiation capacity.
ISSN:1873-5061
1876-7753
DOI:10.1016/j.scr.2022.103000