SSR genetic linkage map construction of pea (Pisum sativum L.) based on Chinese native varieties

Simple sequence repeat (SSR) markers have previously been applied to linkage mapping of the pea (Pisum sativum L.) genome. However, the transferability of existing loci to the molecularly distinct Chinese winter pea gene pool was limited. A novel set of pea SSR markers was accordingly developed. Together with existing SSR sequences, the genome of the G0003973 (winter hardy)×G0005527 (cold sensitive) cross was mapped using 190 F2 individuals. In total, 157 SSR markers were placed in 11 linkage groups with an average interval of 9.7cM and total coverage of 1518cM. The novel markers and genetic linkage map will be useful for marker-assisted pea breeding.