Genomic Analysis of Limosilactobacillus fermentum ATCC 23271, a Potential Probiotic Strain with Anti- Candida Activity
(ATCC 23271) was originally isolated from the human intestine and has displayed antimicrobial activity, primarily against species. Complete genome sequencing and comparative analyses were performed to elucidate the genetic basis underlying its probiotic potential. The ATCC 23271 genome was found to...
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Veröffentlicht in: | Journal of fungi (Basel) 2021-09, Vol.7 (10), p.794 |
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Sprache: | eng |
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Zusammenfassung: | (ATCC 23271) was originally isolated from the human intestine and has displayed antimicrobial activity, primarily against
species. Complete genome sequencing and comparative analyses were performed to elucidate the genetic basis underlying its probiotic potential. The ATCC 23271 genome was found to contain 2,193,335 bp, with 2123 protein-coding sequences. Phylogenetic analysis revealed that the ATCC 23271 strain shares 941 gene clusters with six other probiotic strains of
. Putative genes known to confer probiotic properties have been identified in the genome, including genes related to adhesion, tolerance to acidic pH and bile salts, tolerance to oxidative stress, and metabolism and transport of sugars and other compounds. A search for bacteriocin genes revealed a sequence 48% similar to that of enterolysin A, a protein from
. However, in vitro assays confirmed that the strain has inhibitory activity on the growth of
species and also interferes with their adhesion to HeLa cells. In silico analyses demonstrated a high probability of the protein with antimicrobial activity. Our data reveal the genome features of
ATCC 23271, which may provide insight into its future use given the functional benefits, especially against
infections. |
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ISSN: | 2309-608X 2309-608X |
DOI: | 10.3390/jof7100794 |