Effects of SERPINE1 on proliferation, migration, invasion and apoptosis of colon cancer cells

Objective To explore the expression of serine proteinase inhibitor family E member 1 (SERPINE1) in colon cancer and its effect on the malignant biological behaviors of colon cancer cells. Methods starBase and TISIDB databases were used to analyze the expression of SERPINE1 in 471 patients with colon cancer and 41 paracancerous tissues, and the relationships of its differential expression with clinical stage and prognostic survival were analyzed. The cancer specimens and paracancerous tissues from 5 patients with colon cancer were collected in No. 940 Hospital of Joint Logistic Support Force, and RT-qPCR and Western blotting were employed to detect the expression of SERPINE1 at mRNA and protein levels. After lentiviral vectors of SERPINE1 overexpression and interference were constructed and transfected into colon cancer RKO and LoVo cells. The experimental cells were assessed by RT-qPCR and Western blotting to verify the efficiency of overexpression and interference. Then cell proliferation, migration, invasio