A novel fluorescent probe for detecting hydrogen sulfide in osteoblasts during lipopolysaccharide-mediated inflammation under periodontitis

Periodontitis, one of the most common chronic inflammatory diseases, affects the quality of life. Osteogenesis plays an important role in the disease. There is a connection between hydrogen sulfide (H 2 S) and periodontitis, but according to the study has been published, the precise role of H 2 S in inflammation remains in doubt. The main reason for the lack of research is that H 2 S is an endogenous gasotransmitter, difficult to discern through testing. So, we synthesized a novel fluorescence probe which can detect H 2 S in vitro. By using the novel H 2 S fluorescence probe, we found that H 2 S changes in osteoblasts mainly by cystathionine-γ-lyase, and H 2 S increases under LPS stimulation. H 2 S could be a potential marker for diagnosis of inflammatory diseases of bone, and might help deepen studies of the changes of H 2 S level and promote the progression on the researches about pathogenesis of periodontitis.