Spatial Variation of Bacteria in Surface Waters of Paranaguá and Antonina Bays, Paraná, Brazil
Spatial variability in the concentration of total bacteria, saprophytes and coliforms abundance was investigated in surface waters of Paranaguá and Antonina Bays. Six points along a profile from the entrance of Paranaguá Bay to the innermost part of Antonina Bay, were sampled on February 26, 1997. T...
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Veröffentlicht in: | Brazilian archives of biology and technology 2002-03, Vol.45 (1), p.27-34 |
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Zusammenfassung: | Spatial variability in the concentration of total bacteria, saprophytes and coliforms abundance was investigated in surface waters of Paranaguá and Antonina Bays. Six points along a profile from the entrance of Paranaguá Bay to the innermost part of Antonina Bay, were sampled on February 26, 1997. Temperature, salinity, dissolved oxygen, pH, particulate organic carbon, water transparency and seston were also measured. Determination of total bacterial abundance and biomass were made with the epifluorescent method. Saprophytic bacteria counts were conducted with ZoBell 2216E culture medium with fresh and 32‰ water and the coliform MPN was enumerated with the chromogenic substrate method. Principal component analysis of biotic and abiotic data showed a gradient from the innermost bay to the baymouth bar with an increase in total and halophobic aerobic cultivated bacteria concentration at Antonina Bay. Greatest values of halophilic aerobic cultivated bacteria were found at the bays middle part. The greatest fecal coliform number was observed near Paranaguá City. We concluded that regarding bacterial spatial distribution the outer region of the estuary was highly influenced by the adjacent ocean and that the inner part had typical estuarine characteristics.
A variabilidade espacial da concentração de bactérias heterotróficas totais e cultiváveis, assim como de coliformes totais e fecais foi investigada em águas superficiais das baías de Paranaguá e Antonina. Para tanto foram efetuadas coletas ao longo de seis pontos em um transecto entre a entrada da Baía de Paranaguá e a parte interna da Baía de Antonina. Também foram obtidos dados de temperatura, salinidade, oxigênio dissolvido, pH, matéria orgânica particulada, transparência e seston da água. Para a contagem das heterotróficas totais e a avaliação da biomassa bacteriana foi usada a microscopia de epifluorescência. A contagem de heterotróficas aeróbicas foi efetuada com o meio de cultura ZoBell 2216E, preparado com água destilada (0‰) e água salina (32‰). O número mais provável de coliformes totais e fecais foi avaliado através de meio de cultura cromogênico. A ACP dos dados abióticos e bióticos mostrou um gradiente da parte interna da Baía de Antonina para a parte externa da Baía de Paranaguá, com um aumento da concentração de bactérias totais e de heterotróficas cultiváveis halófobas na Baía de Antonina e valores máximos de halófilas na parte mediana da Baía de Paranaguá. O número máximo de c |
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ISSN: | 1516-8913 1516-8913 1678-4324 |
DOI: | 10.1590/S1516-89132002000100005 |