Protective Effect of Pyridoxine on Lead Nitrate Induced Histomorphological Changes in Rat Liver

Objective: To study the effects of Pyridoxine on lead nitrate generated histological and morphological changesin rat liver.Study Design: It was an experimental study.Place and Duration of Study: The study was carried out at Anatomy Department of College of Physicians andrd st Surgeons, Islamabad, Pakistan from 03rd September, 2019 to 31st December, 2020.Materials and Methods: In this study 90 male rats were used and divided into three groups. Control group Awas kept on normal diet. Experimental group B1 was given lead nitrate and B2 was given lead nitrate andpyridoxine by oral gavage for a period of 2 weeks. All rats were sacrificed after 2 weeks. Results of quantitativevariables such as inflammatory and Kupffer cells were expressed in mean ± S.D. To detect significant differenceamong the groups, ANOVA accompanied by Post Hoc Tukey test was applied and qualitative data such as central vein congestion, steatosis, and necrosis were expressed in percentages and Chi Square test.Results: Exposure to pyridoxine reduces the degenerative changes caused by lead nitrate such as increase inthe number of Kupffer and inflammatory cells. Central vein congestion, steatosis and necrotic foci were alsoreduced with a significant p-value.Conclusion: An ameliorative effect of pyridoxine on the degenerative changes in the liver induced by leadnitrate was proven through a decrease in the number of kupffer and inflammatory cells. Central veincongestion, steatosis and necrotic foci were also reduced significantly. How to cite this: Abbasi S, Khan HM, Khan MY. Protective Effect of Pyridoxine on Lead Nitrate Induced Histomorphological Changes in Rat Liver. Life and Science. 2022; 3(4): 163-167. doi: http://doi.org/10.37185/LnS.1.1.269