Identification and analysis of key genes in association with development and progression of adenocarcinoma at the gastroesophageal junction

Objective To explore the transcriptomic changes of adenocarcinoma at the gastroesophageal junction (ACGEJ) and identify key genes in the occurrence and progression. Methods RNA sequencing data of ACGEJ tumors and adjacent normal tissues samples from 58 patients who were recruited at the Linzhou Cancer Hospital and Linzhou Esophageal Cancer Hospital. The gene expression level of paired ACGEJ and adjacent normal tissue samples were compared to identify dysregulated genes. And the enriched pathways of these genes were analyzed by using gene set variation analysis (GSVA). LASSO regression model was used to test the association between the expression of dysregulated genes and the prognosis of ACGEJ patients, and the clinical features and prognosis associated gene set were applied to establish the nomogram model. Results Through transcriptomic profiling of tumor and non-tumor ACGEJ samples, 737 significantly differentially expression genes (DEGs) in ACGEJ were identified(｜log2FC｜＞1.2, Q＜0.05). The DEGs were related